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ARL13B antibody [HL2173]

Recombinant antibody KO/KD Orthogonal
Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177
Anti-ARL13B antibody [HL2173] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638177
Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177
Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177

Cat. No. GTX638177

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2173

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARL13B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human ARL13B.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor like GTPase 13B , ARL2L1 , JBTS8

Cellular Localization

Cell projection, cilium

Background

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177

GTX638177 WB Image

KO/KD

Non-transfected (–) and transfected (+) RMS 13 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARL13B antibody [HL2173] (GTX638177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARL13B antibody [HL2173] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638177

GTX638177 ICC/IF Image

ARL13B antibody [HL2173] detects ARL13B protein at cytoplasmic vesicles by immunofluorescent analysis.
Sample: RMS-13 cells were fixed in ice-cold MeOH for 5 min.
Green: ARL13B stained by ARL13B antibody [HL2173] (GTX638177) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177

GTX638177 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARL13B antibody [HL2173] (GTX638177) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ARL13B antibody [HL2173] used in Western Blot (WB). GTX638177

GTX638177 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARL13B antibody [HL2173] (GTX638177) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations