GeneTex
United States (US)

ARMET antibody [N1C3]

GTX102060_WB_19011506_689.jpg
GTX102060_39609_IFA_19011506_789.jpg

Cat No. GTX102060

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARMET. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARMET antibody, ARP antibody, MGC142148 antibody, MGC142150 antibody, MANF antibody, mesencephalic astrocyte-derived neurotrophic factor antibody, "arginine-rich, mutated in early stage tumors antibody"

Background

The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102060_WB_19011506_689.jpg

GTX102060 WB Image

Sample(30 μg of whole cell lysate)
A:293T
12% SDS PAGE
GTX102060 diluted at 1:1500

GTX102060_39609_IFA_19011506_789.jpg

GTX102060 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using ARMET(GTX102060) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf