GeneTex
United States (US)

ARPC1A antibody [N1], N-term

GTX104743_39666_IHC_19011506_971.jpg
GTX104743_39666_WB_B_19011506_319.jpg

Cat No. GTX104743

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

ARPC1A transfected 293T lysate

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ARPC1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arc40 antibody, SOP2Hs antibody, SOP2L antibody, ARPC1A antibody, SOP2-like protein antibody, actin-related protein 2/3 complex subunit 1A antibody, actin binding protein (Schizosaccharomyces pombe sop2-like) antibody, "actin related protein 2/3 complex, subunit 1A, 41kDa antibody"

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. [provided by RefSeq]

Database

DATA IMAGES
GTX104743_39666_IHC_19011506_971.jpg

GTX104743 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ARPC1A(GTX104743) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104743_39666_WB_B_19011506_319.jpg

GTX104743 WB Image

Sample (30 ug of whole cell lysate)
A:  Non-transfected 293T lysates
  B:  ARPC1A transfected 293T lysates
10% SDS PAGE
GTX104743 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169