APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.5μg/ml |
| Assay dependent |
| 3-5μg/ml |
|
Note :
IHC-P
Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89775-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1; NP_690601.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin related protein 2/3 complex subunit 2 , ARC34 , PNAS-139 , PRO2446 , p34-Arc
Cellular Localization
Cytoplasm, cytoskeleton , Nucleus
Background
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89775 WB Image
WB analysis of HeLa lysate using GTX89775 ARPC2 antibody, C-term.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89775 IHC-P Image
IHC-P analysis of human spleen using GTX89775 ARPC2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
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GTX89775 ICC/IF Image
ICC/IF analysis of PFA fixed A431 cells using GTX89775 ARPC2 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89775 WB Image
WB analysis of CACO-2 (A), HeLa (B) and Jurkat (C) lysate (35μg protein in RIPA buffer) using GTX89775 ARPC2 antibody, C-term.
Dilution : 0.5μg/ml (HeLa) and 0.1μg/ml (CACO-2, HeLa, and Jurkat)
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GTX89775 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX89775 ARPC2 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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REFERENCE
There are currently no references for ARPC2 antibody, C-term (GTX89775). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARPC2 antibody, C-term (GTX89775). Be the first to share your experience with this product.
