GeneTex
United States (US)

ARPC2 antibody

GTX101984_39911_WB_M_kidney_19011506_403.jpg
GTX101984_WB_19011506_804.jpg
GTX101984_IFA_19011506_287.jpg
GTX101984_39911_IHC_19011506_735.jpg
GTX101984_39911_WB_R_kidney_19011506_467.jpg

Cat No. GTX101984

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , HeLaS3 , mouse kidney , rat kidney

Predict Reactivity

Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARC34 antibody, PNAS-139 antibody, p34-Arc antibody, ARPC2 antibody, ARP2/3 protein complex subunit 34 antibody, actin-related protein 2/3 complex subunit 2 antibody, arp2/3 complex 34 kDa subunit antibody, actin related protein 2/3 complex, subunit 2, 34kDa antibody

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101984_39911_WB_M_kidney_19011506_403.jpg

GTX101984 WB Image

ARPC2 antibody detects ARPC2 protein by western blot analysis.
A. 50 µg mouse kidney lysate/extract
10% SDS-PAGE
ARPC2 antibody (GTX101984) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101984_WB_19011506_804.jpg

GTX101984 WB Image

Sample(30 µg of whole cell lysate)
A:293T
B: HeLa S3(GTX14654)
12% SDS PAGE
GTX101984 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101984_IFA_19011506_287.jpg

GTX101984 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2(GTX101984) antibody at 1:200 dilution.

GTX101984_39911_IHC_19011506_735.jpg

GTX101984 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2(GTX101984) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101984_39911_WB_R_kidney_19011506_467.jpg

GTX101984 WB Image

ARPC2 antibody detects ARPC2 protein by western blot analysis.
A. 50 µg rat kidney lysate/extract
10% SDS-PAGE
ARPC2 antibody (GTX101984) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169