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ARS2 antibody

Anti-ARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119872
Anti-ARS2 antibody used in Western Blot (WB). GTX119872
Anti-ARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119872

Cat No. GTX119872

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , HeLa , HepG2

Predict Reactivity

Mouse, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ARS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720168

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

serrate, RNA effector molecule , ARS2 , ASR2 , serrate

Background

Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity.

Database

DATA IMAGES

Anti-ARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119872

GTX119872 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARS2(GTX119872) antibody at 1:500 dilution.

Anti-ARS2 antibody used in Western Blot (WB). GTX119872

GTX119872 WB Image

ARS2 antibody detects ARS2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
ARS2 antibody (GTX119872) dilution: 1:1000

Anti-ARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX119872

GTX119872 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2(GTX119872) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169