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ARSB antibody, Internal

Anti-ARSB antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88904
Anti-ARSB antibody, Internal used in Western Blot (WB). GTX88904

Cat. No. GTX88904

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ARSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.03-0.1μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres.
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88904-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KLARGHTNGTKPLD, from the internal region of the protein sequence according to NP_000037.2; NP_942002.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arylsulfatase B , ASB , G4S , MPS6

Cellular Localization

Lysosome

Background

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-ARSB antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX88904

GTX88904 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX88904 ARSB antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-ARSB antibody, Internal used in Western Blot (WB). GTX88904

GTX88904 WB Image

WB analysis of human heart lysate using GTX88904 ARSB antibody, Internal.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399