GeneTex
United States (US)

ARSB antibody [N3C3]

Overexpression
GTX102829_39715_20170518_WB_M_placenta_19011506_388.jpg
GTX102829_39715_20160317_WB_19011506_378.jpg
GTX102829_WB_19011506_284.jpg
GTX102829_39715_20170518_WB_R_testis_19011506_380.jpg

Cat No. GTX102829

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HeLa , HepG2 , ARSB tansfected 293T cell , mouse placenta , rat testis

Predict Reactivity

Bovine, Cat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARSB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Arylsulfatase B,Asb,G4S,Mps6,Arsb

Cellular Localization

Lysosome

Background

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102829_39715_20170518_WB_M_placenta_19011506_388.jpg

GTX102829 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody (GTX102829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102829_39715_20160317_WB_19011506_378.jpg

GTX102829 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody [N3C3] (GTX102829) diluted at 1:1000.

GTX102829_WB_19011506_284.jpg

GTX102829 WB Image

Overexpression

Western Blot analysis of ARSB expression in transfected 293T cell line by ARSB polyclonal antibody.
A: Non-transfected lysate.
B: ARSB transfected lysate.
12% SDS PAGE
GTX102829 diluted at 1:500

GTX102829_39715_20170518_WB_R_testis_19011506_380.jpg

GTX102829 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody (GTX102829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169