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ARSB antibody [N3C3]

Overexpression
Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829
Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829
Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829
Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829

Cat. No. GTX102829

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all ARSB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

ARSB transfected 293T cell , HeLa , HepG2 , mouse placenta , rat testis

Predict Reactivity

Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARSB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240482

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arylsulfatase B , ASB , G4S , MPS6

Cellular Localization

Lysosome

Background

Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829

GTX102829 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody (GTX102829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829

GTX102829 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody (GTX102829) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829

GTX102829 WB Image

Overexpression

Western Blot analysis of ARSB expression in transfected 293T cell line by ARSB polyclonal antibody.
A: Non-transfected lysate.
B: ARSB transfected lysate.
12% SDS PAGE
GTX102829 diluted at 1:500

Anti-ARSB antibody [N3C3] used in Western Blot (WB). GTX102829

GTX102829 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody [N3C3] (GTX102829) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169