APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1 μg/ml |
| Assay dependent |
| 10μl |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)[4]This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene[3].
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
~2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.
Purification
Purified immunoglobulin
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
septin 4 , ARTS , BRADEION , C17orf47 , CE5B3 , H5 , MART , PNUTL2 , SEP4 , hCDCREL-2 , hucep-7
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton,Nucleus
Background
Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF beta and, to a lesser extent, by other apoptotic agents, such as TNF alpha and Fas ligand.
Database
Research Area
DATA IMAGES
![Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440](/templates/default/js/lightbox/images/loading.gif "Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440")
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GTX10440 WB Image
WB analysis of 293T cells transfected with ARTS plasmid using GTX10440 ARTS antibody [ARTS51] at 1:4000.
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REFERENCE
There are currently no references for ARTS antibody [ARTS51] (GTX10440). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARTS antibody [ARTS51] (GTX10440). Be the first to share your experience with this product.
![Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440](/upload/website/prouct_img/small/GTX10440/GTX10440_20170605_WB_w_23060120_513.webp "Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440")
![Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440](/upload/website/prouct_img/normal/GTX10440/GTX10440_20170605_WB_w_23060120_513.webp "Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440")