GeneTex
  • Country / Location Selection

United States (US)

ARTS antibody [ARTS51]

Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440

Cat No. GTX10440

Host Mouse
Clonality Monoclonal
Clone Name ARTS51
Isotype IgG2b
Application WB, ICC/IF, IP, ELISA
Reactivity Human
Package
50 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1 μg/ml
ICC/IF Assay dependent
IP 10μl
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

This monoclonal anti-ARTS antibody was used to detect ARTS (Sept4_i2) protein in whole lysates of HeLa cells. HeLa ARTS knockdown (ARTS KD) stable cell line was established by using short hairpin RNAs (shRNAs) which abrogated specifically the endogenous levels of ARTS (Sept4_i2, ~30Kd)[4]This monoclonal Ab is the only currently commercially available antibody directed against the unique C-terminus of ARTS, that can distinguish betweenARTS ( the pro-apoptotic, tumor suppressor, specific XIAP- antagonist) and Sept4_i1 (H5,PNUTL), which is the other product of the SEPT4 gene[3].
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

septin 4 , ARTS , BRADEION , C17orf47 , CE5B3 , H5 , MART , PNUTL2 , SEP4 , hCDCREL-2 , hucep-7

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton,Nucleus

Background

Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF beta and, to a lesser extent, by other apoptotic agents, such as TNF alpha and Fas ligand.

Database

Research Area

DATA IMAGES
Anti-ARTS antibody [ARTS51] used in Western Blot (WB). GTX10440

GTX10440 WB Image

WB analysis of 293T cells transfected with ARTS plasmid using GTX10440 ARTS antibody [ARTS51] at 1:4000.

REFERENCE
There are currently no references for ARTS antibody [ARTS51] (GTX10440). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ARTS antibody [ARTS51] (GTX10440). Be the first to share your experience with this product.
Package List Price ($)
$ 329