WB analysis of insulin-treated LO2 (A), insulin-treated Raw264.7 (B), insulin-treated PC12 (C), UV-treated NIH3T3 (D) whole cell lysates using GTX55118 AS160 / TBC1D4 (phospho Thr642) antibody.

ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX55118 AS160 / TBC1D4 (phospho Thr642) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

IHC-P analysis of formalin fixed human brain tissue section using GTX55118 AS160 / TBC1D4 (phospho Thr642) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)