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AS160 / TBC1D4 (phospho Thr642) antibody

Anti-AS160 / TBC1D4 (phospho Thr642) antibody used in Western Blot (WB). GTX76675

Cat No. GTX76675

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($479)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1mg/ml BSA, 50% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AS160 that contains threonine 642. The sequence is conserved in mouse.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TBC1 domain family member 4 , AS160 , NIDDM5

Cellular Localization

Cytoplasm

Background

This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-AS160 / TBC1D4 (phospho Thr642) antibody used in Western Blot (WB). GTX76675

GTX76675 WB Image

WB (peptide competition) analysis of 3T3-L1 cells (Lane 1-4) or COS cells over-expressing WT AS160 (Lane 5, 6) or mutant AS160 T642A (Lane 7), treated with PI-3-Kinase inhibitor LY294002 (Lane 5) or insulin (Lane 1-4, 6-7) using GTX76675 AS160 / TBC1D4 (phospho Thr642) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf