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ASAH1 antibody

Anti-ASAH1 antibody used in Western Blot (WB). GTX114267
Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114267
Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114267

Cat. No. GTX114267

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ASAH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASAH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623755

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N-acylsphingosine amidohydrolase 1 , AC , ACDase , ASAH , PHP , PHP32 , SMAPME

Cellular Localization

Lysosome

Background

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASAH1 antibody used in Western Blot (WB). GTX114267

GTX114267 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASAH1 antibody (GTX114267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114267

GTX114267 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ASAH1(GTX114267) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114267

GTX114267 IHC-P Image

ASAH1 antibody detects ASAH1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ASAH1 stained by ASAH1 antibody (GTX114267) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

MonserratP?rez-Ram?rez et al. Clinical Neurology and Neurosurgery 2019; (Epub) Pediatric ependymoma: GNAO1, ASAH1, IMMT and IPO7 protein expression and 5-year prognosis correlation.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169