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ASAH1 antibody

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356
Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356
Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356
Anti-ASAH1 antibody used in Western Blot (WB). GTX31356
Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356

Cat No. GTX31356

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ASAH1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human ASAH1.The immunogen is located within amino acids 240 - 290 of ASAH1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

N-acylsphingosine amidohydrolase 1 , AC , ACDase , ASAH , PHP , PHP32 , SMAPME

Cellular Localization

Lysosome,Secreted,Cytoplasm

Background

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356

GTX31356 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31356 ASAH1 antibody.
Dilution : 5 μg/ml

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356

GTX31356 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31356 ASAH1 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356

GTX31356 IHC-P Image

IHC-P analysis of human heart tissue using GTX31356 ASAH1 antibody.
Working concentration : 2.5 μg/ml

Anti-ASAH1 antibody used in Western Blot (WB). GTX31356

GTX31356 WB Image

WB analysis of human heart tissue lysate using GTX31356 ASAH1 antibody.
Working concentration : 1 μg/ml

Anti-ASAH1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31356

GTX31356 IHC-P Image

IHC-P analysis of human heart tissue using GTX31356 ASAH1 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf