APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 - 2 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HEK293 Cell Lysate(GTX27902)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2.The immunogen is located within the last 50 amino acids of ASAH2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
N-acylsphingosine amidohydrolase 2 , BCDase , HNAC1 , LCDase , N-CDase , NCDase
Cellular Localization
Golgi apparatus membrane,Mitochondrion
Background
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
|
GTX31775 WB Image
WB analysis of 293 cell lysate using GTX31775 ASAH2 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
|
REFERENCE
There are currently no references for ASAH2 antibody (GTX31775). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ASAH2 antibody (GTX31775). Be the first to share your experience with this product.