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ASAH2 antibody

Anti-ASAH2 antibody used in Western Blot (WB). GTX31775

Cat No. GTX31775

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2.The immunogen is located within the last 50 amino acids of ASAH2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

N-acylsphingosine amidohydrolase 2 , BCDase , HNAC1 , LCDase , N-CDase , NCDase

Cellular Localization

Golgi apparatus membrane,Mitochondrion

Background

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ASAH2 antibody used in Western Blot (WB). GTX31775

GTX31775 WB Image

WB analysis of 293 cell lysate using GTX31775 ASAH2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339