GeneTex
United States (US)

ASAP1 antibody

GTX12424_20170605_IHC-P_1_18121410_715.jpg
GTX12424_20170605_IHC-P_3_18121410_179.jpg
GTX12424_20170605_IHC-P_2_18121410_578.jpg
GTX12424_20170605_IHC-P_4_18121410_720.jpg
GTX12424_20170605_WB_18121410_426.jpg
GTX12424_20170605_IHC-P_18121410_584.jpg

Cat No. GTX12424

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,500
ICC/IF 1:1,000
IHC-P Assay dependent

Note :

4 μg antibody using 500 μg of Jurkat RIPA lysate
Not tested in other applications.

Calculated MW

130 kDa (doublet band) kDa. ( Note )

Positive Control

rat brain, mouse brain, NIH3T3
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 15 mM sodium azide

Antigen Species

Mouse

Immunogen

synthetic peptide located near the C-terminus of mouse ASAP1 (amino acids 1128-1147) conjugated to KLH.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DDEF1, ZG14P, Centaurin beta4, AV239055, ASAP1, Centaurin beta 4, AMAP1, PAG2, mKIAA1249, 50807, PAP, s19, KIAA1249, Q9ULH1, 13196, 605953

Background

ADP-ribosylation factors (ARFs) family of GTP binding proteins are involved in the regulation of membrane trafficking and actin cytoskeleton. ASAP1 (ARF GAP containing SH3, ANK repeat and PH domains 1, also known as DEF1 or centaurin beta 4) is a 130 kDa, ADP ribosylation factor GTPase activating protein (ARFGAP). ARFs require ARF-GAPs that stimulate their GTPase activity. ASAP1 belongs to a family of related ARF-GAP proteins, consisting of PAP/DEF2, ACAP1, ACAP2, GIT1/CAT1/APP1, and GIT2/CAT2/PKL, which are involved in the regulation of the actin cytoskeleton and cell migration. ASAP1 binds ARF1 and ARF5, with only modest activity toward ARF6. It also binds to other known regulators of actin cytoskeleton, such as tyrosine kinase Src, Crk, FAK and phosphatidylinositol-4,5-bisphosphate (PIP2). ASAP1 is phosphorylated on tyrosine residues in cells expressing activated Src.ASAP1a and ASAP1b are two variants formed by alternative splicing of the ASAP1 gene. ASAP1 localizes to focal adhesions and cycles with focal adhesion proteins when cells are stimulated to move. Overexpression of ASAP1 alters the morphology of focal adhesions, blocks cell spreading and formation of dorsal ruffles, and prevents the efficient organization of paxillin and FAK in focal adhesions. On the other hand, a mutation disrupting GAP activity of ASAP1 or overexpression of a truncated variant of ASAP1 that fails to bind FAK has a reduced effect on inhibition of cell spreading. By directly interacting with both ARFs and focal adhesion proteins such as paxillin and FAK, ASAP1 plays an important role in the regulation of focal adhesion assembly and cytoskeletal remodeling.
Show More +
DATA IMAGES
GTX12424_20170605_IHC-P_1_18121410_715.jpg

GTX12424 IHC-P Image

IHC-P analysis of rat brain tissue using GTX12424 ASAP1 antibody at 0.1 μg/mL.

GTX12424_20170605_IHC-P_3_18121410_179.jpg

GTX12424 IHC-P Image

IHC-P analysis of blood vessels in mouse brain tissue using GTX12424 ASAP1 antibody at 0.2 μg/mL.

GTX12424_20170605_IHC-P_2_18121410_578.jpg

GTX12424 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX12424 ASAP1 antibody at 0.2 μg/mL.

GTX12424_20170605_IHC-P_4_18121410_720.jpg

GTX12424 IHC-P Image

IHC-P analysis of blood vessels in mouse tongue tissue using GTX12424 ASAP1 antibody at 0.2 μg/mL.

GTX12424_20170605_WB_18121410_426.jpg

GTX12424 WB Image

WB analysis of mouse brain (Lanes A, C) and rat brain (Lane B) cytosolic fractions using GTX12424 ASAP1 antibody.
Lane A and B : Antibody dilution 1: 2,500
Lane C : 1: 2,500 + 10 mg/mL ASAP1 immunizing peptide

GTX12424_20170605_IHC-P_18121410_584.jpg

GTX12424 IHC-P Image

IHC-P analysis of rat brain tissue using GTX12424 ASAP1 antibody at 0.1 μg/mL.

Package List Price ($)
$ 289