GeneTex
United States (US)

ASB4 antibody [N1C3]

Anti-ASB4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107345
Anti-ASB4 antibody [N1C3] used in Western Blot (WB). GTX107345
Anti-ASB4 antibody [N1C3] used in Western Blot (WB). GTX107345

Cat No. GTX107345

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

A431 , Mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ankyrin Repeat And Socs Box Containing 4,Asb-4,Asb4

Background

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but some of the full length sequences are not known. [provided by RefSeq]

Database

DATA IMAGES
Anti-ASB4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107345

GTX107345 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ASB4(GTX107345) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASB4 antibody [N1C3] used in Western Blot (WB). GTX107345

GTX107345 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ASB4 antibody [N1C3] (GTX107345) diluted at 1:500.

Anti-ASB4 antibody [N1C3] used in Western Blot (WB). GTX107345

GTX107345 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107345 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169