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ASB5 antibody [N1C3]

Anti-ASB5 antibody [N1C3] used in Western Blot (WB). GTX107132
Anti-ASB5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107132
Anti-ASB5 antibody [N1C3] used in Western Blot (WB). GTX107132

Cat. No. GTX107132

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ASB5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , HeLaS3 , HepG2 , mouse muscle

Predict Reactivity

Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASB5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949694

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ankyrin repeat and SOCS box containing 5 , ASB-5

Background

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but their full length sequences are not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASB5 antibody [N1C3] used in Western Blot (WB). GTX107132

GTX107132 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:HeLa S3(GTX14654)
C:Hep G2 (GTX27900)
10% SDS PAGE
GTX107132 diluted at 1:1000

Anti-ASB5 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107132

GTX107132 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using ASB5(GTX107132) antibody at 1:500 dilution.

Anti-ASB5 antibody [N1C3] used in Western Blot (WB). GTX107132

GTX107132 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ASB5 antibody [N1C3] (GTX107132) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169