Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ASB5 antibody [N1C3] (GTX107132) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed Hep G2, using ASB5(GTX107132) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:HeLa S3(GTX14654)
C:Hep G2 (GTX27900)
10% SDS PAGE
GTX107132 diluted at 1:1000