ASCC2 antibody [N3C2], Internal

Cat. No. GTX116950

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all ASCC2 products

Datasheet
Document

View product citations for antibody GTX116950 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating signal cointegrator 1 complex subunit 2 , ASC1p100 , p100

Background

Enhances NF-kappa-B, SRF and AP1 transactivation.

Database

Gene ID: 84164 ASCC2
UniProt: Q9H1I8 ASCC2

Research Area

DATA IMAGES

GTX116950 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ASCC2(GTX116950) antibody at 1:500 dilution.

GTX116950 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ASCC2(GTX116950) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116950 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
7.5% SDS PAGE
GTX116950 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty