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ASCL1 antibody

Anti-ASCL1 antibody used in Western Blot (WB). GTX01090

Cat. No. GTX01090

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all ASCL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Human, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HKSAPKQVKRQRS, from the internal region of the protein sequence according to NP_004307.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ASCL1 , ASH1 , HASH1 , MASH1 , achaetescute family bHLH transcription factor 1 , bHLHa46

Cellular Localization

Nucleus

Background

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ASCL1 antibody used in Western Blot (WB). GTX01090

GTX01090 WB Image

WB analysis of mouse (Lane A) and rat (Lane B) lung lysates (35μg protein in RIPA buffer) using GTX01090 ASCL1 antibody.
Dilution : 1μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399