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ASCL1 antibody

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129189

Cat No. GTX129189

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat, Dog
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , SH-SY5Y , SH-SY5Y nuclear extract , PC-12 , Rat2

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

achaete-scute family bHLH transcription factor 1 , ASH1 , HASH1 , MASH1 , bHLHa46

Cellular Localization

Nucleus

Background

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

Various whole cell and SH-SY5Y nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 26482608

Anti-ASCL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129189

GTX129189 IHC-P Image

ASCL1 antibody detects ASCL1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ASCL1 antibody (GTX129189) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

ASCL1 antibody detects ASCL1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with ASCL1 antibody (GTX129189) diluted by 1:1000

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000.

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000.

Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129189

GTX129189 ICC/IF Image

ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASCL1 stained by ASCL1 antibody (GTX129189) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169