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ASCL1 antibody

Orthogonal
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189
Anti-ASCL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129189
Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

Cat. No. GTX129189

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all ASCL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , PC-12 , Rat2 , SH-SY5Y nuclear extract

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885918

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

achaete-scute family bHLH transcription factor 1 , ASH1 , HASH1 , MASH1 , bHLHa46

Cellular Localization

Nucleus

Background

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000.

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

Orthogonal

Various whole cell and SH-SY5Y nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX129189) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 26482608

Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129189

GTX129189 ICC/IF Image

ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASCL1 stained by ASCL1 antibody (GTX129189) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

ASCL1 antibody detects ASCL1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with ASCL1 antibody (GTX129189) diluted by 1:1000

Anti-ASCL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129189

GTX129189 IHC-P Image

ASCL1 antibody detects ASCL1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ASCL1 antibody (GTX129189) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASCL1 antibody used in Western Blot (WB). GTX129189

GTX129189 WB Image

The data was published in the 2020 in Nat Commun. PMID: 31953400

REFERENCE

Application Reference

Fushimi A et al. Mol Cancer Res 2022; 20 (9) : 1379-1390 Dependence on the MUC1-C Oncoprotein in Classic, Variant, and Non-neuroendocrine Small Cell Lung Cancer.
Application : WB
Reactivity : Human
Luan Z et al. Carcinogenesis 2021; (Epub) MUC1-C Dictates Neuroendocrine Lineage Specification in Pancreatic Ductal Adenocarcinomas.
Application : WB
Reactivity : Human
Yasumizu Y et al. Nat Commun 2020; 11 (1) : 338 MUC1-C regulates lineage plasticity driving progression to neuroendocrine prostate cancer.
Application : WB
Reactivity : Human
Nakano R et al. PLoS One 2020; 15 (3) : e0229892 All-trans retinoic acid induces reprogramming of canine dedifferentiated cells into neuron-like cells.
Application : WB
Reactivity : Dog
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169