GeneTex
  • Country / Location Selection

United States (US)

ASCL1 antibody

Anti-ASCL1 antibody used in Western Blot (WB). GTX133105
Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133105

Cat No. GTX133105

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

SH-SY5Y , SH-SY5Y nuclear extract

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse ASCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

achaete-scute family bHLH transcription factor 1,AI225900,ASH1,Mash1,bHLHa46

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
Anti-ASCL1 antibody used in Western Blot (WB). GTX133105

GTX133105 WB Image

Various whole cell and SH-SY5Y nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX133105) diluted at 1:5000.
The observed M.W. is based on the publication: PMID: 26482608

Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133105

GTX133105 ICC/IF Image

ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASCL1 stained by ASCL1 antibody (GTX133105) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
There are currently no references for ASCL1 antibody (GTX133105). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ASCL1 antibody (GTX133105). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169