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ASCL1 antibody

Orthogonal
Anti-ASCL1 antibody used in Western Blot (WB). GTX133105
Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133105

Cat. No. GTX133105

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all ASCL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

SH-SY5Y nuclear extract

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse ASCL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886828

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

achaete-scute family bHLH transcription factor 1 , AI225900 , ASH1 , Mash1 , bHLHa46

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-ASCL1 antibody used in Western Blot (WB). GTX133105

GTX133105 WB Image

Orthogonal

SH-SY5Y whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ASCL1 antibody (GTX133105) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASCL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133105

GTX133105 ICC/IF Image

ASCL1 antibody detects ASCL1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASCL1 stained by ASCL1 antibody (GTX133105) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169