ASFV p54 antibody [GT1075]

Cat. No. GTX635691

GTX635691 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

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GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX635691 [GT1075])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

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GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

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GTX635691 Lateral Flow Image

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GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

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GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection:
HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 μg/mL)
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

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GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT1075] (GTX635691) (5 μg/mL)
Detection:
ASFV p54 antibody (GTX135175) (1 μg/mL)

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GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro, 0.2 μg/ml) by lateral flow assay.

Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml). Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).

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GTX635691 IHC-P Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX635691 ICC/IF Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

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GTX635691 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1289] (GTX636703) Detection : ASFV p54 antibody [GT1075] (GTX635691)

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GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1287] (GTX636701) Detection : ASFV p54 antibody [GT1075] (GTX635691)

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This ASFV p54 antibody [GT1075] (GTX635691) is a mouse monoclonal antibody validated for detection of ASFV p54 by WB, ICC/IF, IHC-P, ELISA, Lateral Flow, and Sandwich ELISA. Reactivity has been shown in African swine fever virus.

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1075

Isotype

IgG1

Applications

WB ICC/IF IHC-P ELISA Lateral Flow Sandwich ELISA

Reactivity

African swine fever virus

See all ASFV p54 products