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ASFV p54 antibody [GT1075]

Anti-ASFV p54 antibody [GT1075] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691
Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Western Blot (WB). GTX635691
Anti-ASFV p54 antibody [GT1075] used in IHC (Paraffin sections) (IHC-P). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

Cat No. GTX635691

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1075

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

African swine fever virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636701 / GTX636703 , Detection: GTX635691

Sandwich ELISA
Capture : GTX635690, Detection : GTX635691 or Capture : GTX635691, Detection : GTX135175.
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888563

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

African swine fever virus p54

Database

Research Area

DATA IMAGES

Anti-ASFV p54 antibody [GT1075] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635691

GTX635691 ICC/IF Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 protein (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml)._x005F_x000B_
Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).

Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691

GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection:
HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 μg/mL)
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691

GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT1075] (GTX635691) (5 μg/mL)
Detection:
ASFV p54 antibody (GTX135175) (1 μg/mL)

Anti-ASFV p54 antibody [GT1075] used in Western Blot (WB). GTX635691

GTX635691 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [GT1075] used in IHC (Paraffin sections) (IHC-P). GTX635691

GTX635691 IHC-P Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 protein, His tag by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 protein, His tag by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1287] (GTX636701)
Detection : ASFV p54 antibody [GT1075] (GTX635691)

REFERENCE

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REVIEW

ASFV p54 antibody [GT1075] Cat No. GTX635691

Rating ( Average 5 based on 2 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 21-Apr-2022
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : 1ng African swine fever virus p54 protein, GTX135175-pro
Figure Note : Detection of African swine fever virus p54 protein (partial) by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody [HL1287] (GTX636701) or ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : ASFV p54 protein
Amount used : 0.2 μg/ml
Figure Note : Detection of ASFV p54 protein (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml).
Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).
Package List Price ($)
$ 379
$ 169