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ASFV p54 antibody [GT853]

Anti-ASFV p54 antibody [GT853] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635690
Anti-ASFV p54 antibody [GT853] used in Western Blot (WB). GTX635690
Anti-ASFV p54 antibody [GT853] used in IHC (Paraffin sections) (IHC-P). GTX635690
Anti-ASFV p54 antibody [GT853] used in Lateral Flow (Lateral Flow). GTX635690
Anti-ASFV p54 antibody [GT853] used in ELISA (ELISA). GTX635690
Anti-ASFV p54 antibody [GT853] used in ELISA (ELISA). GTX635690

Cat No. GTX635690

Host

Mouse

Clonality

Monoclonal

Clone Name

GT853

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

African swine fever virus
Package
100 μl ($379),
25 μl ($169)
PRODUCT

Summary

ASFV p54 antibody detects envelope protein p54 of African swine fever virus. Protein p54, encoded by the E183L gene, is a 20 kDa type I transmembrane protein that localizes to envelope precursors derived from the host ER. p54 is involved in intracellular microtubule-dependent transport of viral capsid toward viral factories, and is detected on membrane-like structures within viral factories and mature extracellular virions. This is a valuable mouse monoclonal antibody for research into this very contagious hemorrhagic disease of pigs.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635690, Detection : GTX135175 / GTX635691
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888562

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

African swine fever virus p54

Database

Research Area

DATA IMAGES

Anti-ASFV p54 antibody [GT853] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635690

GTX635690 ICC/IF Image

ASFV p54 antibody [GT853] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [GT853] used in Western Blot (WB). GTX635690

GTX635690 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT853] (GTX635690) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [GT853] used in IHC (Paraffin sections) (IHC-P). GTX635690

GTX635690 IHC-P Image

ASFV p54 antibody [GT853] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green: ASFV p54 stained by ASFV p54 antibody [GT853] (GTX635690) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASFV p54 antibody [GT853] used in Lateral Flow (Lateral Flow). GTX635690

GTX635690 Lateral Flow Image

Detection of ASFV p54 protein (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml)._x005F_x000B_
Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).

Anti-ASFV p54 antibody [GT853] used in ELISA (ELISA). GTX635690

GTX635690 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection: ASFV p54 antibody (GTX135175) (1 μg/mL).

Anti-ASFV p54 antibody [GT853] used in ELISA (ELISA). GTX635690

GTX635690 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection:
HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 μg/mL)
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

REFERENCE

There are currently no references for ASFV p54 antibody [GT853] (GTX635690). Be the first to share your publications with this product.

REVIEW

ASFV p54 antibody [GT853] Cat No. GTX635690

Rating ( Average 5 based on 1 users reviews)
Application
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : ASFV p54 protein
Amount used : 0.2 μg/ml
Figure Note : Detection of ASFV p54 protein (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml).
Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).
Package List Price ($)
$ 379
$ 169