ASFV p54 antibody [HL1218]

Cat. No. GTX636562

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1218
Isotype	IgG
Application	WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	African swine fever virus
Package	100 μl ($399), 25 μl ($169)

See all ASFV p54 products

Datasheet

View product citations for antibody GTX636562 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:500000
ICC/IF	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Lateral Flow Capture : GTX635690 / GTX635691 / GTX636563 / GTX636700 / GTX636701, Detection : GTX636562 Sandwich ELISA Capture : GTX636562, Detection : GTX636563. Please notice that GTX636563 needs to be conjugated to HRP when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636562 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1218] (GTX636562) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636562 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1218] (GTX636562) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1219] (GTX636563) (0.1 μg/mL)
Please notice that GTX636563 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636562. Please contact us for custom HRP-conjugated antibody.

GTX636562 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635690 [GT853])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636562 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636700 [HL1286])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636562 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636563 [HL1219])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636562 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636562 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (10 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636562 ICC/IF Image

ASFV p54 antibody [HL1218] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1218] (GTX636562) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

ASFV p54 antibody [HL1218]

Cat. No. GTX636562

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX636562 WB Image

GTX636562 ELISA Image

GTX636562 Lateral Flow Image

GTX636562 Lateral Flow Image

GTX636562 Lateral Flow Image

GTX636562 Lateral Flow Image

GTX636562 Lateral Flow Image

GTX636562 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.