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ASFV p54 antibody [HL1286]

Recombinant antibody
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Western Blot (WB). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Lateral Flow (Lateral Flow). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Lateral Flow (Lateral Flow). GTX636700

Cat. No. GTX636700

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1286

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

African swine fever virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636700, Detection : GTX636562 / GTX636703

Sandwich ELISA
Capture : GTX636700, Detection: GTX636703 / GTX636701 or Capture : GTX636703 / GTX636701, Detection: GTX636700.
Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54, His Tag (GTX137950-pro) using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636700

GTX636700 ICC/IF Image

ASFV p54 antibody [HL1286] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1286] (GTX636700) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) (1 μg/mL)
Please notice that GTX636700 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in Western Blot (WB). GTX636700

GTX636700 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1286] (GTX636700) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in Lateral Flow (Lateral Flow). GTX636700

GTX636700 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636700 [HL1286])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1286] used in Lateral Flow (Lateral Flow). GTX636700

GTX636700 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636700 [HL1286])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

REFERENCE

There are currently no references for ASFV p54 antibody [HL1286] (GTX636700). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ASFV p54 antibody [HL1286] (GTX636700). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations