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ASFV p54 antibody [HL1286]

Recombinant antibody
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Western Blot (WB). GTX636700
Anti-ASFV p54 antibody [HL1286] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636700
Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

Cat. No. GTX636700

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1286

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 African swine fever virus
Package
100 μl ($399),
25 μl ($169)
See all ASFV p54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636700, Detection: GTX636703 / GTX636701 or Capture : GTX636703 / GTX636701, Detection: GTX636700.
Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910048

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54, His Tag (GTX137950-pro) using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) (1 μg/mL)
Please notice that GTX636700 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1286] used in Western Blot (WB). GTX636700

GTX636700 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1286] (GTX636700) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [HL1286] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636700

GTX636700 ICC/IF Image

ASFV p54 antibody [HL1286] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1286] (GTX636700) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [HL1286] used in ELISA (ELISA). GTX636700

GTX636700 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations