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ASFV p54 antibody [HL1287]

Recombinant antibody
Anti-ASFV p54 antibody [HL1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Western Blot (WB). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

Cat. No. GTX636701

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1287

Isotype

IgG

Application

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

Reactivity

African swine fever virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636701, Detection : GTX635691 / GTX636562 / GTX636703

Sandwich ELISA
Capture : GTX636701, Detection: GTX636703 / GTX636700 or Capture : GTX636703 / GTX636700, Detection: GTX636701.
Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-ASFV p54 antibody [HL1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636701

GTX636701 ICC/IF Image

ASFV p54 antibody [HL1287] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (10 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX635691 [GT1075])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1289] (GTX636703) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636703. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) (1 μg/mL)
Please notice that GTX636700 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in Western Blot (WB). GTX636701

GTX636701 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ) ASFV p54 antibody [HL1287] (GTX636701) Detection : ASFV p54 antibody [GT1075] (GTX635691)

REFERENCE

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REVIEW

ASFV p54 antibody [HL1287] Cat. No. GTX636701

Rating ( Average 5 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 11-Jul-2023
Score :
Application Tested : ICC/IF
Sample : ASFV p54 protein expressed in Vero cells
Fixation : 4% PFA in PBS, RT/20min
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, RT/0.5hr
Primary Antibody : 1:10000, 4°C, 12Hr
Date : Anonymous submitted on 21-Apr-2022
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : ASFV p54 recombinant protein, partial
Amount used : 1ng
Figure Note : Detection of African swine fever virus p54 protein (partial) by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody [HL1287] (GTX636701) or ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)
Package List Price ($)
$ 399
$ 169
Custom Conjugations