ASFV p54 antibody [HL1287]

Name: ASFV p54 antibody [HL1287]
Brand: GeneTex
SKU: GTX636701
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

Anti-ASFV p54 antibody [HL1287] used in Western Blot (WB). GTX636701

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

Anti-ASFV p54 antibody [HL1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636701

Cat. No. GTX636701

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1287
Isotype	IgG
Application	WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	African swine fever virus
Package	100 μl ($399), 25 μl ($169)

See all ASFV p54 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:100000
ICC/IF	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Lateral Flow Capture : GTX636701, Detection : GTX635691 / GTX636562 / GTX636703 Sandwich ELISA Capture : GTX636701, Detection: GTX636703 / GTX636700 or Capture : GTX636703 / GTX636700, Detection: GTX636701. Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910049

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1289] (GTX636703) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636703. Please contact us for custom HRP-conjugated antibody.

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) (1 μg/mL)
Please notice that GTX636700 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

GTX636701 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636701 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (10 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX635691 [GT1075])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636701 ICC/IF Image

ASFV p54 antibody [HL1287] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636701 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ) ASFV p54 antibody [HL1287] (GTX636701) Detection : ASFV p54 antibody [GT1075] (GTX635691)

REFERENCE

There are currently no references for ASFV p54 antibody [HL1287] (GTX636701). Be the first to share your publications with this product.

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REVIEW

ASFV p54 antibody [HL1287] Cat. No. GTX636701

Rating

( Average 5 based on 2 users reviews)

Application

Immunocytochemistry/ Immunofluorescence(ICC/IF)		( Average 5 based on 1 users reviews)
Lateral Flow(Lateral Flow)		( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 11-Jul-2023

Score :

Application Tested : ICC/IF

Sample : ASFV p54 protein expressed in Vero cells

Fixation : 4% PFA in PBS, RT/20min

Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, RT/0.5hr

Primary Antibody : 1:10000, 4°C, 12Hr

Date : Anonymous submitted on 21-Apr-2022

Score :

Application Tested : Lateral Flow

Sample Species : ASFV

Sample : ASFV p54 recombinant protein, partial

Amount used : 1ng

Figure Note : Detection of African swine fever virus p54 protein (partial) by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody [HL1287] (GTX636701) or ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)

Submit a Review

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty Custom Conjugations

ASFV p54 antibody [HL1287]

Cat. No. GTX636701

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX636701 ELISA Image

GTX636701 ELISA Image

GTX636701 ELISA Image

GTX636701 ELISA Image

GTX636701 WB Image

GTX636701 Lateral Flow Image

GTX636701 Lateral Flow Image

GTX636701 Lateral Flow Image

GTX636701 Lateral Flow Image

GTX636701 ICC/IF Image

GTX636701 Lateral Flow Image

REFERENCE

REVIEW

ASFV p54 antibody [HL1287] Cat. No. GTX636701

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.