ASFV p54 antibody [HL1289]

Name: ASFV p54 antibody [HL1289]
Brand: GeneTex
SKU: GTX636703
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ASFV p54 antibody [HL1289] used in ELISA (ELISA). GTX636703

Anti-ASFV p54 antibody [HL1289] used in Western Blot (WB). GTX636703

Anti-ASFV p54 antibody [HL1289] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636703

Anti-ASFV p54 antibody [HL1289] used in Lateral Flow (Lateral Flow). GTX636703

Cat. No. GTX636703

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1289
Isotype	IgG
Application	WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA
Reactivity	African swine fever virus
Package	100 μl ($399), 25 μl ($169)

See all ASFV p54 products

Datasheet

View product citations for antibody GTX636703 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:100000
ICC/IF	Assay dependent
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
Note : Lateral Flow Capture : GTX635690 / GTX635691 / GTX636563 / GTX636700 / GTX636701, Detection : GTX636703 Sandwich ELISA Capture : GTX636700 / GTX636701, Detection: GTX636703 or Capture : GTX636703, Detection: GTX636701 / GTX636700. Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910050

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

UniProt: Q65194

Research Area

DATA IMAGES

GTX636703 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1289] (GTX636703) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636703. Please contact us for custom HRP-conjugated antibody.

GTX636703 ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54, His Tag (GTX137950-pro) using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

GTX636703 ELISA Image

GTX636703 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1289] (GTX636703) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636703 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

GTX636703 ICC/IF Image

ASFV p54 antibody [HL1289] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1289] (GTX636703) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636703 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635690 [GT853])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636703 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636703 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636700 [HL1286])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636703 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

GTX636703 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1289] (GTX636703)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

GTX636703 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636563 [HL1219])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

REFERENCE

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REVIEW