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ASFV p54 antibody

Anti-ASFV p54 antibody used in ELISA (ELISA). GTX135175
Anti-ASFV p54 antibody used in ELISA (ELISA). GTX135175
Anti-ASFV p54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135175
Anti-ASFV p54 antibody used in Western Blot (WB). GTX135175

Cat. No. GTX135175

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, Sandwich ELISA

Reactivity

 African swine fever virus
Package
100 μl ($399),
25 μl ($169)
See all ASFV p54 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX635690 / GTX635691, Detection: GTX135175

Please notice that GTX135175 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690 / GTX635691. Please contact us for custom HRP-conjugated antibody.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

African swine fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

African swine fever virus p54

Research Area

DATA IMAGES

Anti-ASFV p54 antibody used in ELISA (ELISA). GTX135175

GTX135175 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection: ASFV p54 antibody (GTX135175) (1 μg/mL)

Anti-ASFV p54 antibody used in ELISA (ELISA). GTX135175

GTX135175 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [GT1075] (GTX635691) (5 μg/mL)
Detection: ASFV p54 antibody (GTX135175) (1 μg/mL)

Anti-ASFV p54 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135175

GTX135175 ICC/IF Image

ASFV p54 antibody detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody (GTX135175) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-ASFV p54 antibody used in Western Blot (WB). GTX135175

GTX135175 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody (GTX135175) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169