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ASGPR1 antibody [N1C3]

Anti-ASGPR1 antibody [N1C3] used in Western Blot (WB). GTX122674
Anti-ASGPR1 antibody [N1C3] used in Western Blot (WB). GTX122674
Anti-ASGPR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122674

Cat. No. GTX122674

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ASGPR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Huh-7 , HepG2

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human ASGPR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177321

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

asialoglycoprotein receptor 1 , ASGPR , ASGPR1 , CLEC4H1 , HL-1

Cellular Localization

Membrane

Background

Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specifically removed from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to the cell surface and the ligand is transported to the lysosomes for degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASGPR1 antibody [N1C3] used in Western Blot (WB). GTX122674

GTX122674 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] (GTX122674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASGPR1 antibody [N1C3] used in Western Blot (WB). GTX122674

GTX122674 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASGPR1 antibody [N1C3] (GTX122674) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASGPR1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122674

GTX122674 ICC/IF Image

ASGPR1 antibody [N1C3] detects ASGPR1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASGPR1 stained by ASGPR1 antibody [N1C3] (GTX122674) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Povero D et al. Sci Rep 2022; 12 (1) : 3027 Protein and miRNA profile of circulating extracellular vesicles in patients with primary sclerosing cholangitis.
Reactivity : Human
Kobayashi Y et al. Front Physiol 2021; 12 : 693007 Protein Composition of Circulating Extracellular Vesicles Immediately Changed by Particular Short Time of High-Intensity Interval Training Exercise.
Application : WB
Reactivity : Human
Povero D et al. PLoS One 2014; 9 (12) : e113651 Circulating extracellular vesicles with specific proteome and liver microRNAs are potential biomarkers for liver injury in experimental fatty liver disease.
Application : WB
Reactivity : Mouse
Yeh TH et al. J Drug Target 2014; Intracellular delivery of cytochrome c by galactosylated albumin to hepatocarcinoma cells.
Application : WB
Reactivity : Human
Chen YH et al. J Proteomics 2012; 77 : 111-28 Effect of high glucose on secreted proteome in cultured retinal pigmented epithelium cells: its possible relevance to clinical diabetic retinopathy.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169