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ASIC3 antibody

Anti-ASIC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54807
Anti-ASIC3 antibody used in Western Blot (WB). GTX54807

Cat. No. GTX54807

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all ASIC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 (Intracellular, N-terminus) of rat ASIC3 (Accession  : O35240).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887890

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acid sensing ion channel subunit 3 , Accn3

Cellular Localization

Cytoplasm,Cell membrane

Background

subunit that forms a proton gated Na(+) channel; may play a role in prolonged pain perception [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-ASIC3 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54807

GTX54807 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54807 ASIC3 antibody. Cell nuclei were stained with DAPI (blue).
Panel A : ASIC3 staining.
Panel B : ASIC1 staining in same section (green).
Panel C : Merge of the two images shows some co-localization (arrow).
Dilution : 1:400

Anti-ASIC3 antibody used in Western Blot (WB). GTX54807

GTX54807 WB Image

WB analysis of rat DRG lysate using GTX54807 ASIC3 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

Application Reference

Ren P et al. Neurosci Lett 2018; (Epub) up-regulation of asic3 expression by β-*.
Application : WB
Reactivity : Rat
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REVIEW

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Application Reference
Ren P et al. Neurosci Lett 2018; (Epub) up-regulation of asic3 expression by β-*.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639