ASK1 antibody [2E4]

Cat. No. GTX60456

Host	Mouse
Clonality	Monoclonal
Clone Name	2E4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

See all ASK1 products

Datasheet
Document

View product citations for antibody GTX60456 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

155 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Database

Gene ID: 4217 MAP3K5
UniProt: Q99683 MAP3K5

Research Area

DATA IMAGES

GTX60456 WB Image

WB analysis of human ASK1 (AA: 922-1108) recombinant protein using GTX60456 ASK1 antibody [2E4].

GTX60456 ELISA Image

ELISA analysis of antigen using GTX60456 ASK1 antibody [2E4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60456 IHC-P Image

IHC-P analysis of breast cancer tissue (left) and prostate tissue (right) using GTX60456 ASK1 antibody [2E4].

GTX60456 FACS Image

FACS analysis of HeLa cells using GTX60456 ASK1 antibody [2E4].
Green : ASK1
Purple : negative control

GTX60456 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60456 ASK1 antibody [2E4].
Green : ASK1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty