APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human MAP3K5 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5
Cellular Localization
Cytoplasm,Endoplasmic reticulum
Background
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX60456 WB Image
WB analysis of human ASK1 (AA: 922-1108) recombinant protein using GTX60456 ASK1 antibody [2E4].
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GTX60456 ELISA Image
ELISA analysis of antigen using GTX60456 ASK1 antibody [2E4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60456 IHC-P Image
IHC-P analysis of breast cancer tissue (left) and prostate tissue (right) using GTX60456 ASK1 antibody [2E4].
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GTX60456 FACS Image
FACS analysis of HeLa cells using GTX60456 ASK1 antibody [2E4]. Green : ASK1 Purple : negative control
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GTX60456 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60456 ASK1 antibody [2E4]. Green : ASK1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
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REFERENCE
There are currently no references for ASK1 antibody [2E4] (GTX60456). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ASK1 antibody [2E4] (GTX60456). Be the first to share your experience with this product.