GeneTex
United States (US)

ASK1 antibody [C2C3-2], C-term

GTX107921_39995_IHC_19011507_727.jpg
GTX107921_39995_20180727_WB_B_19011507_597.jpg
GTX107921_39995_WB_M_lung_19011507_984.jpg
GTX107921_WB_19011507_257.jpg

Cat No. GTX107921

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

HeLaS3 , mouse lung , MAP3K5-transfected 293T

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ASK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase Kinase 5,Ask1,Mapkkk5,Mekk5,Map3K5

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107921_39995_IHC_19011507_727.jpg

GTX107921 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASK1(GTX107921) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX107921_39995_20180727_WB_B_19011507_597.jpg

GTX107921 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ASK1 antibody [C2C3-2], C-term (GTX107921) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107921_39995_WB_M_lung_19011507_984.jpg

GTX107921 WB Image

ASK1 antibody [C2C3-2], C-term detects MAP3K5 protein by Western blot analysis.
A. 50 µg mouse lung lysate/extract
5 % SDS-PAGE
ASK1 antibody [C2C3-2], C-term (GTX107921) dilution: 1:1000

GTX107921_WB_19011507_257.jpg

GTX107921 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
5% SDS PAGE
GTX107921 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169