APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 μg/mL |
| 10 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A-431 Cell Slide(GTX25525) , SW1353 Cell Lysate(GTX27915)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1.The sequence is different from that of mouse by last two amino acids.The immunogen is located within the last 50 amino acids of ASK1.
Purification
Purified by DEAE chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5
Cellular Localization
Cytoplasm,Endoplasmic reticulum
Background
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX31637 ICC/IF Image
ICC/IF analysis of A431 cells using GTX31637 ASK1 antibody.
Working concentration : 10 μg/ml
|
|
|
GTX31637 WB Image
WB analysis of SW1353 cell lysate using GTX31637 ASK1 antibody.
Working concentration : 1:500
|
|
|
GTX31637 ICC/IF Image
ICC/IF analysis of A431 cells using GTX31637 ASK1 antibody.
Working concentration : 20 μg/ml
|
REFERENCE
There are currently no references for ASK1 antibody (GTX31637). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ASK1 antibody (GTX31637). Be the first to share your experience with this product.
