GeneTex
United States (US)

ASK1 antibody

Anti-ASK1 antibody used in Western Blot (WB). GTX31637
Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31637
Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31637

Cat No. GTX31637

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

A-431 Cell Slide(GTX25525) , SW1353 Cell Lysate(GTX27915)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1.The sequence is different from that of mouse by last two amino acids.The immunogen is located within the last 50 amino acids of ASK1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase Kinase 5,Ask1,Mapkkk5,Mekk5,Map3K5

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-ASK1 antibody used in Western Blot (WB). GTX31637

GTX31637 WB Image

WB analysis of SW1353 cell lysate using GTX31637 ASK1 antibody.
Working concentration : 1:500

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31637

GTX31637 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31637 ASK1 antibody.
Working concentration : 10 μg/ml

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31637

GTX31637 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31637 ASK1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 329