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ASK1 antibody

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31727
Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31727
Anti-ASK1 antibody used in Western Blot (WB). GTX31727

Cat No. GTX31727

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
ICC/IF 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Positive Control

A-431 Cell Slide(GTX25525) , A431 Cell Lysate(GTX27909) , SW1353 Cell Lysate(GTX27915) , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.The immunogen is located within amino acids 1260 - 1310 of ASK1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31727

GTX31727 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31727 ASK1 antibody.
Working concentration : 2 μg/ml

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31727

GTX31727 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31727 ASK1 antibody.
Working concentration : 20 μg/ml

Anti-ASK1 antibody used in Western Blot (WB). GTX31727

GTX31727 WB Image

WB analysis of SW1353 cell lysate using GTX31727 ASK1 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339