*Optimal dilutions/concentrations should be determined by the researcher.
|0.5 - 2 μg/mL
Not tested in other applications.
A-431 Cell Slide(GTX25525) , A431 Cell Lysate(GTX27909) , SW1353 Cell Lysate(GTX27915) , 3T3/BALB Cell Slide
PBS with 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.The immunogen is located within amino acids 1260 - 1310 of ASK1.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
GTX31727 ICC/IF Image
ICC/IF analysis of A431 cells using GTX31727 ASK1 antibody.
Working concentration : 2 μg/ml
GTX31727 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX31727 ASK1 antibody.
Working concentration : 20 μg/ml
GTX31727 WB Image
WB analysis of SW1353 cell lysate using GTX31727 ASK1 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml