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ASK1 antibody

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50471
Anti-ASK1 antibody used in Western Blot (WB). GTX50471
Anti-ASK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50471

Cat. No. GTX50471

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ASK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.81~85 (G-S-S-V-G) derived from human ASK1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50471

GTX50471 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50471 ASK1 antibody.

Anti-ASK1 antibody used in Western Blot (WB). GTX50471

GTX50471 WB Image

WB analysis of extracts from MDA-MB-435 cells using GTX50471 ASK1 antibody with or without blocking peptide.

Anti-ASK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50471

GTX50471 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50471 ASK1 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399