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ASK1 antibody

Anti-ASK1 antibody used in Western Blot (WB). GTX50471
Anti-ASK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50471
Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50471

Cat No. GTX50471

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.81~85 (G-S-S-V-G) derived from human ASK1.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 5 , ASK1 , MAPKKK5 , MEKK5

Cellular Localization

Cytoplasm,Endoplasmic reticulum

Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ASK1 antibody used in Western Blot (WB). GTX50471

GTX50471 WB Image

WB analysis of extracts from MDA-MB-435 cells using GTX50471 ASK1 antibody with or without blocking peptide.

Anti-ASK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX50471

GTX50471 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50471 ASK1 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-ASK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50471

GTX50471 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50471 ASK1 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf