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ASL antibody

KO/KD
Anti-ASL antibody used in Western Blot (WB). GTX109750
Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109750
Anti-ASL antibody used in Western Blot (WB). GTX109750
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX109750

Cat. No. GTX109750

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ASL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Molt-4 , HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949696

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

argininosuccinate lyase , ASAL

Background

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASL antibody used in Western Blot (WB). GTX109750

GTX109750 WB Image

KO/KD

Wild-type (WT) and ASL knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASL antibody (GTX109750) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109750

GTX109750 ICC/IF Image

ASL antibody detects ASL protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASL protein stained by ASL antibody (GTX109750) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ASL antibody used in Western Blot (WB). GTX109750

GTX109750 WB Image

Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
10% SDS PAGE
GTX109750 diluted at 1:1000

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX109750

GTX109750 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using ASL(GTX109750) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169