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ASL antibody

Anti-ASL antibody used in Western Blot (WB). GTX113629
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629
Anti-ASL antibody used in Western Blot (WB). GTX113629
Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629
Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

Cat No. GTX113629

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

argininosuccinate lyase,ASAL

Background

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ASL antibody used in Western Blot (WB). GTX113629

GTX113629 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASL antibody (GTX113629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

GTX113629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASL antibody used in Western Blot (WB). GTX113629

GTX113629 WB Image

ASL antibody detects ASL protein by western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
10% SDS-PAGE
ASL antibody (GTX113629) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629

GTX113629 ICC/IF Image

ASL antibody detects ASL protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASL protein stained by ASL antibody (GTX113629) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629

GTX113629 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(GTX113629) antibody at 1:200 dilution.

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

GTX113629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169