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ASM antibody [ARC62108]

Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580
Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580
Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580
Anti-ASM antibody [ARC62108] used in IHC (Paraffin sections) (IHC-P). GTX04580
Anti-ASM antibody [ARC62108] used in IHC (Paraffin sections) (IHC-P). GTX04580

Cat. No. GTX04580

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 ARC62108

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all ASM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 0.05% BSA, 50% glycerol

Preservative

0.05% proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1(NP_000534.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sphingomyelin phosphodiesterase 1 , ASM , ASMASE , NPD

Cellular Localization

Lysosome , Secreted

Background

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580

GTX04580 WB Image

WB analysis of various samples using GTX04580 ASM antibody [ARC62108].
Loading : 25 μg
Dilution : 1:1000

Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580

GTX04580 WB Image

WB analysis of HeLa cells with/without ASM KO samples using GTX04580 ASM antibody [ARC62108].
Loading : 25 μg
Dilution : 1:1000

Anti-ASM antibody [ARC62108] used in Western Blot (WB). GTX04580

GTX04580 WB Image

WB analysis of SK-MEL-28 whole cell lysate using GTX04580 ASM antibody [ARC62108].
Loading : 25 μg
Loading : 1:1000

Anti-ASM antibody [ARC62108] used in IHC (Paraffin sections) (IHC-P). GTX04580

GTX04580 IHC-P Image

IHC-P analysis of human liver tissue using GTX04580 ASM antibody [ARC62108].
Antigen retrieval : 10 mM citrate buffer (pH 6.0)
Dilution : 1:100

Anti-ASM antibody [ARC62108] used in IHC (Paraffin sections) (IHC-P). GTX04580

GTX04580 IHC-P Image

IHC-P analysis of human brain tissue using GTX04580 ASM antibody [ARC62108].
Antigen retrieval : 10 mM citrate buffer (pH 6.0)
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399