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ASPH antibody, N-term

Anti-ASPH antibody, N-term used in Western Blot (WB). GTX45856
Anti-ASPH antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45856

Cat No. GTX45856

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

1x PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human ASPH

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

aspartate beta-hydroxylase , AAH , BAH , CASQ2BP1 , FDLAB , HAAH , JCTN , junctin

Background

This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-ASPH antibody, N-term used in Western Blot (WB). GTX45856

GTX45856 WB Image

WB analysis of HepG2 cells using GTX45856 ASPH antibody at 2.5μg/ml.
Lane A: marker
Lane B: HepG2 cells

Anti-ASPH antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45856

GTX45856 IHC-P Image

IHC-P analysis of human kidney tissue using GTX45856 ASPH antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379