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ASS1 antibody [2B10]

Anti-ASS1 antibody [2B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60549
Anti-ASS1 antibody [2B10] used in Western Blot (WB). GTX60549
Anti-ASS1 antibody [2B10] used in ELISA (ELISA). GTX60549
Anti-ASS1 antibody [2B10] used in Flow cytometry (FACS). GTX60549
Anti-ASS1 antibody [2B10] used in Western Blot (WB). GTX60549

Cat. No. GTX60549

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($409)
See all ASS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ASS1 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887978

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

argininosuccinate synthase 1 , ASS , CTLN1

Cellular Localization

Cytoplasm, cytosol

Background

The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-ASS1 antibody [2B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60549

GTX60549 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60549 ASS1 antibody [2B10].
Green : ASS1
Blue: DRAQ5 fluorescent DNA dye

Anti-ASS1 antibody [2B10] used in Western Blot (WB). GTX60549

GTX60549 WB Image

WB analysis of A431 (1), RAJI (2), L1210 (3), MOLT4 (4), Jurkat (5), A549 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60549 ASS1 antibody [2B10].

Anti-ASS1 antibody [2B10] used in ELISA (ELISA). GTX60549

GTX60549 ELISA Image

ELISA analysis of antigen using GTX60549 ASS1 antibody [2B10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-ASS1 antibody [2B10] used in Flow cytometry (FACS). GTX60549

GTX60549 FACS Image

FACS analysis of Jurkat cells using GTX60549 ASS1 antibody [2B10].
Green : ASS1
Red : negative control

Anti-ASS1 antibody [2B10] used in Western Blot (WB). GTX60549

GTX60549 WB Image

WB analysis of human ASS1 (AA: 40-236) recombinant protein using GTX60549 ASS1 antibody [2B10].

REFERENCE

Application Reference

Lee YZ et al. Pharmacol Res 2019; 152 : 104581 Tylophorine-based compounds are therapeutic in rheumatoid arthritis by targeting the caprin-1 ribonucleoprotein complex and inhibiting expression of associated c-Myc and HIF-1帢.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409