GeneTex
United States (US)

ASXL1 antibody

GTX127284_41024_CT_WB_B_18121410_280.jpg
GTX127284_41024_20150821_IHC-P_18121410_240.jpg

Cat No. GTX127284

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Positive Control

human ASXL1-transfected 293T cells

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:26385183)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ASXL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Asxl Transcriptional Regulator 1,Bops,Mds,Asxl1

Cellular Localization

Nucleus

Background

This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX127284_41024_CT_WB_B_18121410_280.jpg

GTX127284 WB Image

ASXL1 antibody detects ASXL1 protein by western blot analysis.
A. 50 µg 293T whole cell lysate/extract
B. 50 µg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
ASXL1 antibody (GTX127284) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127284_41024_20150821_IHC-P_18121410_240.jpg

GTX127284 IHC-P Image

ASXL1 antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
ASXL1 antibody (GTX127284) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

ASXL1 antibody Cat No. GTX127284

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : A.50ug 293T whole cell lysate/extract, B.50ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169