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ASXL1 antibody

KO/KD Overexpression
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284
Anti-ASXL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127284

Cat. No. GTX127284

Reference 3
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ASXL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

Positive Control

GFP-human ASXL1-transfected 293T cells

Product Note

Knockdown/Knockout validation was supported by references (PMID:26385183)

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ASXL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885631

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ASXL transcriptional regulator 1 , BOPS , MDS

Cellular Localization

Nucleus

Background

This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASXL1 antibody used in Western Blot (WB). GTX127284

GTX127284 WB Image

Whole cell extract (30 Aμg) was separated by 5% SDS-PAGE, and the membrane was blotted with ASXL1 antibody (GTX127284) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASXL1 antibody used in Western Blot (WB). GTX127284

GTX127284 WB Image

Overexpression

ASXL1 antibody detects ASXL1 protein by western blot analysis.
A. 50 μg 293T whole cell lysate/extract
B. 50 μg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
ASXL1 antibody (GTX127284) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASXL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127284

GTX127284 IHC-P Image

ASXL1 antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
ASXL1 antibody (GTX127284) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Moon S et al. Cell Death Dis 2018; 9 (11) : 1118 Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis.
Application : WB
Reactivity : Mouse
Youn HS et al. Sci Rep 2017; 7 (1) : 5198 Asxl1 deficiency in embryonic fibroblasts leads to cellular senescence via impairment of the AKT-E2F pathway and Ezh2 inactivation.
Application : WB
Reactivity : Mouse
Moon S et al. Biochem Biophys Res Commun 2015; 466 (3) : 560-6 Role of Asxl1 in kidney podocyte development via its interaction with Wtip.
Application : WB
Reactivity : Mouse
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REVIEW

ASXL1 antibody Cat. No. GTX127284

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284
Date : Anonymous submitted on 17-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : A.50ug 293T whole cell lysate/extract, B.50ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169