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ATF1 (phospho Ser63) antibody

Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420
Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420
Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420

Cat No. GTX130420

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , 293T (25 μg/ml anisomycin treatment for 30 min) , A431 , A431(200 ng/ml EGF treatment for 30 min)

Product Note

This antibody is raised against human ATF1 phosphorylated at Ser63, and it also may detect the phosphorylated CREB protein due to sequence homology.

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human ATF1 (phospho Ser63). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886259

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

activating transcription factor 1 , EWS-ATF1 , FUS/ATF-1 , TREB36

Cellular Localization

Nucleus

Background

This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420

GTX130420 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000.

Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420

GTX130420 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATF1 (phospho Ser63) antibody used in Western Blot (WB). GTX130420

GTX130420 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody (GTX130420) diluted at 1:5000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169