APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Product Note
This antibody is raised against human ATF2 phosphorylated at Ser112. Based on sequence homology, it is predicted to react with mouse/rat ATF2 when phosphorylated at the corresponding residues - ATF2 Ser94.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 112 or 94 (D-L-S(p)-P-L) derived from human ATF2.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
activating transcription factor 2 , CRE-BP1 , CREB-2 , CREB2 , HB16 , TREB7
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion outer membrane
Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
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GTX50687 WB Image
WB analysis of extracts from HUVEC cells untreated or treated with Anisomycin using GTX50687 ATF2 (phospho Ser112) antibody.
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GTX50687 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50687 ATF2 (phospho Ser112) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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REFERENCE
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REVIEW
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