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ATF2 (phospho Thr69) antibody

Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in ELISA (ELISA). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805
Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX00805

Cat No. GTX00805

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ATF2 around the phosphorylation site of Thr69.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ATF2 , CREB2 , CREBP1 , HB16 , TREB7 , activating transcription factor 2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX00805

GTX00805 WB Image

WB analysis of rat and mouse brain tissue lysates using GTX00805 ATF2 (phospho Thr69) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00805

GTX00805 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells with LPS treatment for 4hrs using GTX00805 ATF2 (phospho Thr69) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse brain tissue by using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in ELISA (ELISA). GTX00805

GTX00805 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00805 ATF2 (phospho Thr69) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat lung tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human ganstric cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human ganstric cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat ganstric tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat lung tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat ganstric tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in IHC (Paraffin sections) (IHC-P). GTX00805

GTX00805 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200

Anti-ATF2 (phospho Thr69) antibody used in Western Blot (WB). GTX00805

GTX00805 WB Image

WB analysis of various sample lysates using GTX00805 ATF2 (phospho Thr69) antibody.
Lane 1 : Heat-shock treated MCF7 cells, blocked with antigen-specific peptides
Lane 2 : Heat-shock treated MCF7 cells
Lane 3 : Heat-shock treated HeLa cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf