APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human ATF2 around the phosphorylation site of Thr69.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATF2 , CREB2 , CREBP1 , HB16 , TREB7 , activating transcription factor 2
Cellular Localization
Nucleus,Cytoplasm,Mitochondrion outer membrane
Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
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GTX00805 WB Image
WB analysis of various sample lysates using GTX00805 ATF2 (phospho Thr69) antibody.
Lane 1 : Heat-shock treated MCF7 cells, blocked with antigen-specific peptides
Lane 2 : Heat-shock treated MCF7 cells
Lane 3 : Heat-shock treated HeLa cells
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GTX00805 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 WB Image
WB analysis of rat and mouse brain tissue lysates using GTX00805 ATF2 (phospho Thr69) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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GTX00805 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of human ganstric cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of human pancreas tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of mouse brain tissue by using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00805 ATF2 (phospho Thr69) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX00805 IHC-P Image
IHC-P analysis of human ganstric cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 ICC/IF Image
ICC/IF analysis of PFA-fixed HeLa cells with LPS treatment for 4hrs using GTX00805 ATF2 (phospho Thr69) antibody.
Red : Primary antibody
Green : Tubulin
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of rat lung tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of rat ganstric tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
|
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GTX00805 IHC-P Image
IHC-P analysis of rat lung tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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GTX00805 IHC-P Image
IHC-P analysis of rat ganstric tissue using GTX00805 ATF2 (phospho Thr69) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:200
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REFERENCE
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REVIEW
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