Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF2 (phospho Thr71) antibody [GT1275] (GTX03187) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37ºC for 30 minutes using GTX03187 ATF2 (phospho Thr71) antibody [GT1275].
Dilution : 1:1000
Loading : 25μg per lane