GeneTex

  • Country / Location Selection

United States (US)

ATF2 (phospho Thr71) antibody [GT1275]

Recombinant antibody Orthogonal
Anti-ATF2 (phospho Thr71) antibody [GT1275] used in Western Blot (WB). GTX03187
Anti-ATF2 (phospho Thr71) antibody [GT1275] used in Western Blot (WB). GTX03187

Cat. No. GTX03187

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1275

Isotype

 IgG

Application

 WB

Reactivity

 Mouse, Rat
Package
100 μl ($419)
See all ATF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T71 of human ATF2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 2 , CRE-BP1 , CREB-2 , CREB2 , HB16 , TREB7

Cellular Localization

Nucleus , Cytoplasm , Mitochondrion outer membrane

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ATF2 (phospho Thr71) antibody [GT1275] used in Western Blot (WB). GTX03187

GTX03187 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF2 (phospho Thr71) antibody [GT1275] (GTX03187) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATF2 (phospho Thr71) antibody [GT1275] used in Western Blot (WB). GTX03187

GTX03187 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37ºC for 30 minutes using GTX03187 ATF2 (phospho Thr71) antibody [GT1275].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for ATF2 (phospho Thr71) antibody [GT1275] (GTX03187). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ATF2 (phospho Thr71) antibody [GT1275] (GTX03187). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 419