GeneTex

  • Country / Location Selection

United States (US)

ATF2 (phospho Thr71) antibody

Orthogonal
Anti-ATF2 (phospho Thr71) antibody used in IHC (Paraffin sections) (IHC-P). GTX24736
Anti-ATF2 (phospho Thr71) antibody used in Western Blot (WB). GTX24736
Anti-ATF2 (phospho Thr71) antibody used in IHC (Paraffin sections) (IHC-P). GTX24736
Anti-ATF2 (phospho Thr71) antibody used in Western Blot (WB). GTX24736

Cat. No. GTX24736

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all ATF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
IHC-P 1:20
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Chemically synthesized phosphopeptide derived from a region of human ATF2 that contains threonine 71

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 2 , CRE-BP1 , CREB-2 , CREB2 , HB16 , TREB7

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ATF2 (phospho Thr71) antibody used in IHC (Paraffin sections) (IHC-P). GTX24736

GTX24736 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ATF2 (phospho Thr71) antibody used in Western Blot (WB). GTX24736

GTX24736 WB Image

Orthogonal

WB (peptide competition) analysis of Jurkat cells treated with 10 μg/mL anisomycin for 60 minutes using GTX24736 ATF2 (phospho Thr71) antibody prior incubated with the phosphopeptide immunogen (Lane 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), or a generic phosphothreonine-containing peptide (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

Anti-ATF2 (phospho Thr71) antibody used in IHC (Paraffin sections) (IHC-P). GTX24736

GTX24736 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24736 ATF2 (phospho Thr71) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ATF2 (phospho Thr71) antibody used in Western Blot (WB). GTX24736

GTX24736 WB Image

Orthogonal

WB analysis of whole cell extracts (30 μg lysate) of U-87 MG (Lane 1), U-87 MG treated with 25 μg/mL anisomycin for 30 minutes (Lane 2), K562 (Lane 3), K562 treated with 25 μg/mL anisomycin for 30 minutes (Lane 4) using GTX24736 ATF2 (phospho Thr71) antibody.
Dilution : 1-2 μg/ml

REFERENCE

There are currently no references for ATF2 (phospho Thr71) antibody (GTX24736). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ATF2 (phospho Thr71) antibody (GTX24736). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399