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ATF2 (phospho Thr73) antibody

Anti-ATF2 (phospho Thr73) antibody used in IHC (Paraffin sections) (IHC-P). GTX55047
Anti-ATF2 (phospho Thr73) antibody used in Western Blot (WB). GTX55047

Cat. No. GTX55047

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ATF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of ATF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887911

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 2 , CRE-BP1 , CREB-2 , CREB2 , HB16 , TREB7

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion outer membrane

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ATF2 (phospho Thr73) antibody used in IHC (Paraffin sections) (IHC-P). GTX55047

GTX55047 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX55047 ATF2 (phospho Thr73) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-ATF2 (phospho Thr73) antibody used in Western Blot (WB). GTX55047

GTX55047 WB Image

WB analysis of UV-treated HeLa (A), NIH3T3 (B), PC12 (C) whole cell lysates using GTX55047 ATF2 (phospho Thr73) antibody.

REFERENCE

Application Reference

Tran TT et al. Proteomics 2017; (Epub) quantitative phosphoproteome analysis of *-induced apoptosis in jurkat t cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399