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ATF3 antibody [7F1B10]

Anti-ATF3 antibody [7F1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02812
Anti-ATF3 antibody [7F1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02812
Anti-ATF3 antibody [7F1B10] used in Flow cytometry (FACS). GTX02812
Anti-ATF3 antibody [7F1B10] used in Western Blot (WB). GTX02812
Anti-ATF3 antibody [7F1B10] used in Western Blot (WB). GTX02812

Cat. No. GTX02812

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F1B10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($419)
See all ATF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:200-1:1000
FACS 1:200-1:400
ELISA 1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ATF3 (AA: 1-181) expressed in E.Coli.

Purification

Purified IgG1

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 3

Cellular Localization

Nucleus

Background

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Database

Research Area

DATA IMAGES

Anti-ATF3 antibody [7F1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02812

GTX02812 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX02812 ATF3 antibody [7F1B10].
Green : Primary antibody
Blue : DRAQ5 fluorescent DNA dye
Red : Actin filaments

Anti-ATF3 antibody [7F1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02812

GTX02812 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX02812 ATF3 antibody [7F1B10].
Green : Primary antibody
Blue : DRAQ5 fluorescent DNA dye
Red : Actin filaments

Anti-ATF3 antibody [7F1B10] used in Flow cytometry (FACS). GTX02812

GTX02812 FACS Image

FACS analysis of A549 cells using GTX02812 ATF3 antibody [7F1B10].
Green : Primary antibody
Red : Negative control

Anti-ATF3 antibody [7F1B10] used in Western Blot (WB). GTX02812

GTX02812 WB Image

WB analysis of HEK293 (Lane 1) and ATF3 (AA: 1-181)-hIgGFc transfected HEK293 (Lane 2) cell lysate using GTX02812 ATF3 antibody [7F1B10].

Anti-ATF3 antibody [7F1B10] used in Western Blot (WB). GTX02812

GTX02812 WB Image

WB analysis of human ATF3 (AA: 1-181) recombinant protein using GTX02812 ATF3 antibody [7F1B10].

REFERENCE

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REVIEW

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Package List Price ($)
$ 419