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ATF3 antibody

KO/KD Orthogonal
Anti-ATF3 antibody used in Western Blot (WB). GTX129120
Anti-ATF3 antibody used in Western Blot (WB). GTX129120
Anti-ATF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129120

Cat. No. GTX129120

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ATF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HCT116 (UVC-treated 10 J/m2 and recover for 8 hr) , HeLa

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ATF3 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885899

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 3

Cellular Localization

Nucleus

Background

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ATF3 antibody used in Western Blot (WB). GTX129120

GTX129120 WB Image

KO/KD

Wild-type (WT) and ATF3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATF3 antibody (GTX129120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATF3 antibody used in Western Blot (WB). GTX129120

GTX129120 WB Image

Orthogonal

ATF3 antibody detects ATF3 protein by western blot analysis.
A HCT116 (untreated)
B HCT116 (UVC-treated 10 J/m2 and recover for 8 hr)
12 % SDS-PAGE
ATF3 antibody (GTX129120) dilution: 1:1000

Anti-ATF3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129120

GTX129120 ICC/IF Image

ATF3 antibody detects ATF3 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells, irradiated (right) or non-irradiated (left) with 100 J/m2 UVC with 8hr recovery, were fixed in 4% paraformaldehyde at RT for 5 min.
Green: ATF3 protein stained by ATF3 antibody (GTX129120) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Qu J et al. Clin Exp Pharmacol Physiol 2015; (Epub) PFKFB3 modulates glycolytic metabolism and alleviates endoplasmic reticulum stress in human osteoarthritis cartilage.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169