ATF4 antibody

Name: ATF4 antibody
Brand: GeneTex
SKU: GTX101943
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-ATF4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101943

Anti-ATF4 antibody used in Western Blot (WB). GTX101943

Cat. No. GTX101943

Reference 15

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all ATF4 products

View product citations for antibody GTX101943 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

HeLa (4ug/ml tunicamycin treatment for 16 hr)

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 4 , CREB-2 , CREB2 , TAXREB67 , TXREB

Cellular Localization

Cytoplasm , Cell membrane , Nucleus

Background

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq]

Database

Gene ID: 468 ATF4
UniProt: P18848 ATF4

Research Area

DATA IMAGES

GTX101943 ICC/IF Image

ATF4 antibody detects ATF4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 2 μg/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATF4 protein stained by ATF4 antibody (GTX101943) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101943 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody (GTX101943) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.